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Streptococcus Agalactiae Selective Agar Base - …

Please refer disclaimer Overleaf. Streptococcus Agalactiae Selective Agar Base M1257 Streptococcus Agalactiae Selective Agar is recommended for selective isolation of Streptococcus agalactiae from dairy

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Text of Streptococcus Agalactiae Selective Agar Base - …

Please refer disclaimer Agalactiae Selective Agar BaseM1257Streptococcus Agalactiae Selective Agar is recommended for selective isolation of Streptococcus agalactiae from **IngredientsGms / LitrePeptic digest of animal pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 940 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT to 45-50 C and add 60 ml defribinated blood and 25ml Staphylococcus -toxin. Mix well and pour into sterile Petri And InterpretationStreptococcus agalactiae is a gram-positive Streptococcus characterized by the presence of group B Lancefield agalactiae exhibits beta haemolysic reaction. On Blood agar plate, it forms zones of haemolysis that are slightly bigger thanthe size of colonies formed. Group B streptococci hydrolyze sodium hippurate and give a positive response in the CAMP agalactiae is also sensitive to bile and will lyse in its presence. Streptococcus Agalactiae Selective Agar was formulated byHauge and Kohler-Ellingsen (1) for the isolation of S. agalactiae , the causative agent of mastitis in between Streptococcus species is done on the basis of esculin hydrolysis seen as dark brown colour dueto formation of an esculin-thallium complex. Thallous sulphate and crystal violet inhibit the accompanying bacterial -toxin attacks the erythrocytes present in the medium in such a way that they may be completely agalactiae is not haemolytic on simple blood agar. Thus S. agalactiae can be distinguished from obligate, non-haemolyzing agalactiae forms dove-blue coloured smooth colonies surrounded by zones of haemolysis. Further identification is doneby using biochemical and serological methods, but primarily by using CAMP test (2).Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with Agar and Clarity of prepared mediumBasal medium forms light purple coloured, clear to slightly opalescent gel. On addition of blood, red coloured opalescent gelforms in Petri platesReactionReaction of w/v aqueous solution at 25 C. pH : ResponseM1257: Cultural characteristics observed after an incubation at 35-37 C for 24-48 LaboratoriesTechnical DataOrganismInoculum(CFU)GrowthRecoveryB lue colonyHaemolysisCultural ResponseEscherichia coli ATCC25922>=10 inhibited0%Enterococcus faecalis ATCC2921250-100good-luxuriant>=50%variab lereactionalphaPseudomonas aeruginosaATCC 27853>=10 inhibited0%Staphylococcus aureusATCC 25923>=10 inhibited0%Streptococcus agalactiaeATCC 1381350-100luxuriant>=50%positivebetaStr eptococcus agalactiaeATCC 2795650-100luxuriant>=50%positivebetaStr eptococcus cremorisATCC 1925750-100luxuriant>=50%variablereactio nalphaStreptococcus pneumoniaeATCC 6301luxuriant>=50%negativealphaStreptoco ccus pyogenesATCCC 1961550-100luxuriant>=50%negativebetaStr eptococcus lactis ATCC19435>=10 inhibited0%Storage and Shelf LifeStore below 30 C in tightly closed container and the prepared medium at 2 - 8 C. Use before expiry date on the Hauge S. T. and u Kohler-Ellingsen J., 1953, Nord. Vet. Med., 5 Christie R., Atkins N. E. and Munch-Petersen E., 1944, Aust. J. Exp. Biol. Med. Sci., 22 : 1 / 201150-100HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

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